A total of 7039 strains of filamentous fungi are preserved in Korean Agricultural Culture Collection (KACC). The 4065 strains (58%) of them, which produce many spores in cultivation on proper media, are preserved with freeze-drying method. They are also preserved with liquid nitrogen and deep-freezer storage in order to minimize loss by death. , , , , , . which are common in surrounding environments, are included in this category. The others which do not produce spores, or produce few spores in vitro, are preserved with liquid nitrogen, deep-freezer and mineral oil storage. , , , , , . are included in this category. The authors also introduced various fungal preservation methods and provided detailed preservation procedures that are used in KACC.