Yeasts are common inhabitants of the phyllosphere, but our knowledge of their diversity in various fruits and
blossoms are limited. We collected different kinds of blossoms and unripened fruits from Hanbat arboretum, Daejeon, Korea at
the year of 2013. Yeasts were isolated by plating of suspensions prepared for collected samples onto YPD medium containing
antibiotics. BLAST searches were subsequently performed for the comparison of the partially determined sequences of D1/D2
domain of 26S rDNA. As a result, we isolated 57 yeast strains of 31 species from 29 different kinds of blossoms and 6 kinds of
fruits samples. We found huge differences in yeast flora depending on the sample collection season.