In this study, we attempted to isolate fungi from soybean fermented foods produced in Sunchang County and to identify from fungal isolates. Ten fungal isolates were identified with
-tubulin gene. According to the sequences of
-tubulin gene, ten fungal isolates were identified as complex. For further identification of the ten of fungal isolates, omtA gene, one gene of the aflatoxin biosynthesis gene cluster, was sequenced and the sequences were compared with those of and strains from the GenBank database. In addition, identification of the ten fungal isolates was further confirmed using the PCR amplicon of and intergenic region, in which a deletion was recognized relative to and . The amplicon size of the ten fungal isolate strains was smaller than those of and , but the same as that of the reference strain. These results indicated that the ten isolates should be identified as . The protease activity in rice koji made with 6, 13, 17, 27, 37 and 38 of strain, respectively was twice higher than that in control. The kojis made with nine of the isolates, respectively, did not produce aflatoxin, suggesting that the strains could possibly be used as starters for soybean products.