ABSTRACT : In this study, a new strain of Lentinula edodes (Shiitake) Poongnyunko was bred from dikaryotic KFRI 490 and
monokaryotic KFRI 536 by Di-mon crossing method. In this study, the productivity was conducted through bed-log and sawdust
substrate cultivation. The Poongnyunko showed significant intensive production from summer to autumn. Optimum temperatures
of fruit-body formation were 17~24. The total amount of fruit-body production during 4 years by bed-log cultivation was 22 kg/
m3, and total yield of fruit-body until the 3rd flush was 648 g/ 2 kg. In case of sawdust substrate cultivation, the mycelial
incubation period was 110~130 days. The suitable temperature and relative humidity were 18 ± 1C and 90 ± 5% respectively.