ABSTRACT : This study focused on isolation of wild yeasts from natural flowers and elucidation of yeast diversity. Wild yeasts
were isolated from various flowers collected from mountains on the islands including Jejudo, Ulleungdo, Yokjido, and Seonyudo
as well as inlands including Gyejoksan, Oseosan, Beakamsan, and Deogyusan in Korea. Isolated yeasts were identified by
comparison of nucleotide sequences for polymerrase chain reaction-amplified D1/D2 region of 26S rDNA or internal transcribed
spacer 1 and 2 including 5.8S rDNA using BLAST. 289 strains belonging to 134 yeast species were isolated. genus
strains were the most frequently isolated species among the identified yeasts. was also frequently isolated.
Twenty three species including aureus were overlapped between those of mountains on islands and inland.
Physiological functionalities such as antioxidant activity, xanthine oxidase inhibitory activity, and tyrosinase inhibitory activity for
the 289 identified yeast strains were investigated using their supernatant and cell-free extracts. The supernatants of Candida sp.
78-J-2 and SY44-6 showed antioxidant activity of 22.5%, and anti-gout xanthine oxidase inhibitory
activity of 49.6%, respectively.