Two fungal strains were isolated from rods of Quercus sp. (NAAS02335) and Pinus (NAAS05299) in Korea.
These strains were identified as by their morphological and mycological characteristics. The optimal growth
temperature of NAAS02335 and NAAS05299 are 25ºC and 30ºC, respectively. Production of cellulase, xylanase, and ligninase
was tested on agar media supplemented dyes or substrates. Production of cellulase and xylanase of NAAS05299 was higher than
those of NAAS02335, however ligninase activity of NAAS02335 was higher than that of NAAS05299. The activities of cellulase,
xylanase, and amylase of strain NAAS05299 were estimated at 6.7~10.2 times higher than that of NAAS02335. Laccase activity
was only estimated by strain NAAS02335. The lignocellulytic enzymes are induced by substrates such as rice straw, wooden chips
of pine, oak, and poplar. The NAAS05299 was able to degrade filter paper completely after 4 weeks of culturing in liquid media
containing a piece of filter paper at 28ºC with continuous shaking. NAAS05299 was able to degrade rice straw, pine chips, and
oak chips after 4 months in solid culture, however NAAS02335 decomposed only rice straw among tested 4 kinds of biomass.