This study focused on the isolation of wild yeasts from the soils of an herb park in Seoul, Korea, and characterization of unrecorded yeasts. Fifteen yeast strains of nine species were isolated and identified. Among them, strains of the genus, such as (2 strains), (two strains), (2 strains), and sp. (2 strains) were dominant. SU14-2, SU6-2 and SU18-3 were also screened as newly recorded yeasts in Korea. SU6-2 formed ascospores and a pseudomycelium, and SU14-2 grew well in the 5% NaCl-containing yeast extract-peptone-dextrose medium.