Twelve unrecorded yeasts, HL9-1, NS19-2, SA4-1, TJ4-3, YB17-2, DD2-5, DM3-5, JW2-3, NS5-1, NS5-7, NS14-2, and SA17-1, were screened among 97 yeasts from soils of Chungcheongnam-do and Daejeon metropolitancity, Korea. These yeasts were oval or ellipsoidal and had a budding system for vegetative reproduction. They grew well in yeast extract-peptone-dextrose (YPD) medium and, in particular, NS14-2 and SA4-1 grew well in 10% NaCl-containing YPD broth. Nine strains, including NS19-2, assimilated xylose and four yeast strains, such as DD2-5, also assimilated lactose. Physiological functionalities of cell-free extracts and supernatants from two halophilic unrecorded yeasts, SA4-1 and NS14-2, were investigated. Cell-free extracts from SA4-1 and NS14-2 exhibited 71.3% and 68.4% antihypertensive angiotensin I-converting enzyme inhibitory activity.