is an edible mushroom that contains a β-glucan called lentinan, which has antitumor and immune-
enhancing properties. In the present study, the β-glucan contents of mushrooms cultivated on sawdust with different
nutritional supplements and fruiting temperatures were measured using a commercial β-glucan assay kit purchased from Megazyme
(Bray, Ireland). The weight loss of sawdust media and the yield of fruiting bodies showed similar trends, but the yield was more
closely associated with the nutritional supplements used than the weight loss of sawdust media was. The β-glucan contents of L.
edodes were 39.5-42.1%, except in the bean curd refuse + CaCl supplementation group (50.4%). Furthermore, the β-glucan
content decreased with increasing temperatures and was 42.4% at a low fruiting temperature.