We evaluated the antioxidant properties of Lentinula edodes upon sawdust bag cultivation with
5 oak substrates: Quercus acutissima, Q. mongolica, Q. serrata, Q. aliena, and Q. variabilis.
We found that the optimal extraction conditions were 70% (v/v) methanol shaken at 150 rpm at
25°C and 150 rpm for 24 h. The methanolic extracts from L. edodes contained high phenolic and
flavonoid contents, they also exhibited stronger antioxidant activities. The total phenolic
contents and total flavonoid contents of the mushroom extracts ranged from 2.37 to 3.12
milligrams of gallic acid equivalents per gram of dried mushroom (mg GAE/g) and 0.48 to 0.48
milligrams of quercetin equivalents per gram of dried mushroom (mg QE/g), respectively. In
addition, the mushroom extracts exhibited 2,2-diphenyl-1-picrylhydrazyl radical scavenging
activity (15.8% to 89.4%) at 2 to 10 mg/mL, ferric reducing antioxidant power (0.153 to 0.425)
at 5 to 20 mg/mL, and reducing power (0.078 to 0.359) at 5 to 20 mg/mL, respectively. Q. aliena
more effectively increased total phenolic contents, total flavonoid contents, and antioxidant
activities than the other oak substrates.