To screen for potent anti-inflammatory compound-producing yeasts, we evaluated nitric oxide
production inhibitory activities in RAW 264.7 macrophage cells using cell-free extracts from
182 non-pathogenic yeasts. YJ36-1 and
YJ34-2 showed high inhibitory activities of 57.4% and 47.0%, respectively. The microbiological
characteristics of these yeasts were investigated. YJ36-1 formed ascospores
and pseudomycelium. This species grew well at 25°C in yeast extract- peptone-dextrose (YPD)
medium, vitamin-free medium, and 5% NaCl-containing YPD medium.
YJ34-2 was an asporogenous yeast and did not form pseudomycelium. This strain also grew
well at 30°C in YPD medium, vitamin-free medium, and 5% NaCl-containing YPD medium.