The anti-inflammatory effects of cell-free extracts from wild yeasts,
YJ34-2 and YJ36-1, caused by the inhibition of nitric oxide (NO) activity
and cytotoxic effects were determined. Cell-free extracts from these two yeast strains had
dose-dependent inhibitory effects on the production of lipopolysaccharide-induced NO and
there were no cytotoxic effects on the treated cells or negative effects on their proliferation.
Their cell-free extracts were also shown to have inhibitory effects on pro-inflammatory cytokines,
such as tumor necrosis factor (TNF)-α and prostaglandin-E2, in a dose-dependent manner.
Maximal inhibitory activity on NO production occurred in cell-free extracts of Meyerozyma
guilliermondii YJ34-2 cultivated at 30°C for 24 hr and YJ36-1 cultivated
at 25°C for 24 hr in the yeast extract-peptone-dextrose (YPD) media.