Characterization of Pyrenophora avenae Isolated from Discolored Black Oat Seeds in Korea
Jung-Hye Choi, Jeomsoon Kim, Hyeonheui Ham, Theresa Lee, Ju-Young Nah, Hyo-Won Choi, Young Kee Lee, Sung Kee Hong**
Microbial Safety Team, National Institute of Agricultural Sciences, Wanju 55365, Korea1 Crop Protection Division, National Institute of Agricultural Sciences, Wanju 55365, Korea2
In January 2017, discolored black oat seeds were found in the storage depot of a farmhouse in Jeongeup. was detected in 45% of the oat seeds surveyed. All isolates obtained from the seeds were identified as based on the sequences of internal transcribed spacer (ITS) rDNA regions and glyceraldehyde 3-phosphate dehydrogenase (GPDH) gene and validated by morphological and cultural characterization. A phylogenetic tree constructed using the ITS and GPDH sequences showed that the Korean isolates of comprise of four genetically distinct groups. Pathogenicity test validated that the fungus is an infectious agent responsible for discolored black seeds and leaf blotch in oat plants. This is the first study report that causes leaf blotch disease of oat in Korea.
1. 1. National Institute of crop Science. Manual of oat cultivation. Wanju: National Institute of crop Science; 2013.
2. 2. Suttie JM, Reynolds SG. Fodder oats: a world overview. Rome: FAO; 2004.
3. 3. Farr DF, Rossman AY. Fungal databases, U.S. National Fungus Collections, ARS, USDA [Internet]. Beltsville: Systematic Mycology and Microbiology Laboratory; 2018 [cited 2018 Dec 12]. Available from: https://nt.ars-grin.gov/fungaldatabases/.
4. 4. Sheridan JE. Drechslera spp. and other seed-borne pathogenic fungi in New Zealand cereals. N Z J Agric Res 1977;20:91-3.
5. 5. Chidambaram P, Mathur SB, Neergaard P. Identification of seed-borneDrechslera species. Friesia 1973;10:165-207.
6. 6. Chi MH, Park SY, Lee YH. A quick and safe method for fungal DNA extraction. Plant Pathol J 2009;25:108-11.
7. 7. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
8. 8. Berbee ML, Pirseyedi M, Hubbard S.Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3- phosphate dehydrogenase gene sequences. Mycologia 1999;91:964-77.
9. 9. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876-82.
10. 10. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9.
12. 12. Gough FJ, McDaniel ME. Occurrence of oat leaf blotch in Texas in 1973. Plant Dis Report 1974;58:80-1.
13. 13. Harder DE, Haber S. Oat diseases and pathologic techniques. In: Marshall HG, Sorrells ME, editors. Oat science and technology. Madison: American Society of Agronomy; Crop Science Society of America; 1992. p. 307-402.