The goal of this study was to elucidate wild yeast diversity of Geumgang midstream near Sejong metropolitan autonomous city, Korea. Thirty-seven strains of 32 species of wild yeasts were isolated from 43 water and soil samples under the Bulti bridge of Sejong city, Korea. Seven yeasts of each spp. and spp. were the predominant species isolated from samples near the Bulti bridge. SW048 (NNIBRFG9314), SW013 (NNIBRFG9310), SW015 (NNIBRFG9316), SW085 (NNIBRFG9326) and SW008 (NNIBRFG9309) represented novel yeast strains found in Korea for the first time. All of these previously unrecorded yeasts, except for SW015 had ascospores and grew well in yeast extract-peptone-dextrose (YPD), yeast extract-malt extract (YM) and potato-extrose (PD) media. SW085 grew well in vitamin-free medium and SW048, which was a halotolerant wild yeast, grew well YPD medium containing 5 % NaCl. Twenty-six strains representing eight species of wild yeast were isolated from 22 water and soil samples under the Haetmuri bridge of Sejong city, Korea. (12 strains) and (11 strains) were the predominant isolates from samples near the Haetmuri bridge. HB060 (NNIBRFG9317), HB014 (NNIBRFG9332), Xenoramularia neerlandica HB039 (NNIBRFG9335), norvegica HB315 (NNIBRFG9306), HB316 (NNIBRFG9305), GB014 (NNIBRFG9307), and GB015 (NNIBRFG9308) represented novel yeast strains recorded in Korea for the first time. O. kilbournensis HB060 and HB039 did not form ascospores or pseudo-mycelia. All of these previously unrecorded yeasts, except HB014 and HB039, grew well in vitamin-free medium, and HB315 and GB015, which were halotolerant wild yeasts, which grew well in YPD medium containing 5 % NaCl.